PUBLICATIONS

Evaluation of antiepileptic properties of herbal mix of different
combinations by PTZ-induced mouse model

P.S. Venkatesan , S. Sundaresan, M. Eswarya, M. Madhavaselvi, R. Renuka

Abstract:

Background: Epilepsy is a neurological disorder characterized by convulsive seizures. Between 50 and 70 million people worldwide are impacted, and safer medications with superior anticonvulsant qualities and greater accessibility are still needed. Antiepileptic drug (AED) side effects continue to be a significant concern despite advances in pharmacotherapy, as they can lower quality of life and adherence. Herbal medicines are becoming more and more popular as complementary and alternative therapies as a result.

Methods: Using a mouse seizure model caused by PTZ (Pentylenetetrazole) of dose 120 mg/kg.b.wt, this study examines the anticonvulsant efficacy of a traditional medicinal plant called Withania somnifera, Matricaria recutita and Evolvulus alsinoides which are commonly called as Ashwagandha, Chamomile and Morning glory respectively. The herb was mixed with 1.5 % Carboxymethyl cellulose (CMC) and given to mice with various dosage levels and in combination.

Results: The study demonstrates that Evolvulus alsinoides, Withania somnifera, and Matricaria recutita, possesses significant anticonvulsant properties in a PTZ-induced seizure model in mice. Combination of all three showed highest latency of 168.25 ± 30.4s and seizure period of 3.82 ± 7.65 s.

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Evaluation of Anti-Epileptic Properties of Ashwagandha and Chamomile by
PTZ-Induced Mouse Model

Dr. P.S. Venkatesan, M. Eswarya and M. Madhavaselvi

Abstract:

Ayurvedic treatments are used in various types of neurological disorders. One of the common neurological disorders is Epilepsy. Withania somnifera and Matricaria recutita are well known for their medicinal properties, such as antimicrobial, antioxidant, anti-inflammatory, and antiepileptic properties. There are also studies examining the properties of Withania somnifera and Matricaria recutita. This study aims to prove the antiepileptic properties of Withania somnifera and Matricaria recutita extracts by testing them on epilepsy-induced Swizz albino mice. Five groups of male Swizz albino mice of 40±5g of weight, each consisting of 5 mice. The drugs were administered at a dose level of 100 mg/kg b.wt each for a week. The inducing method was used to induce epilepsy. Pentylenetetrazol (PTZ) is injected, which acts as a GABA-A receptor antagonist. It stimulates the nerves and causes seizures. The first group acted as a control, the second group as a reference control, with Diazepam, and the remaining groups were dosed with the herbal compound orally before PTZ injection (120 mg/kg b.wt). The evaluation of the latency period and the seizure period was done. The results were obtained and analyzed with ANOVA. Chamomile and Diazepam showed longer latency periods of 102±42.64 s and 103.5±20.50 s, respectively. In the seizure period, the combination group showed good results, having fewer jerks in the clonic period, less than 10 s of the tonic period, and less than 8 s recovery. This showed the anti-epilepsy activity of the herbal extracts of Withania somnifera and Matricaria recutita.

Keywords: Withania somnifera, Matricaria recutita, Epilepsy, Seizures, Mice, Pentylenetetrazol.

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Evaluation of anti-epileptic properties of Evolvulus alsinoides by pentylenetetrazole-induced mouse model

P. S. Venkatesan*, M. Eswarya, M. Madhavaselvi

Abstract:

Background: A common neurological condition that affects people of all ages, epilepsy is defined by recurring seizures that have serious negative effects on the nervous system, cognition, psychology, and social interactions. Anti-epileptic drug (AED) side effects continue to be a significant concern despite advances in pharmacotherapy, as they can lower quality of life and adherence. Herbal medicines are becoming more and more popular as complementary and alternative therapies as a result.

Methods: Using a mouse seizure model caused by PTZ (Pentylenetetrazole), this study examines the anticonvulsant efficacy of a traditional medicinal plant called Evolvulus alsinoides, often known as dwarf slender morning glory. The herb was mixed with 1.5% CMC and given to mice with various dosage levels.

Results: The effects of various doses of morning glory extract (50 mg/kg to 400 mg/kg body weight) on latency period and seizure duration were compared to those of a control group and a diazepam-treated group. In latency period, 300 mg/kg. b. wt group showed a long latency period of 222.5±47.68 (p<0.05) when comparing to other groups. Morning glory of dose 400 mg/kg b. wt eliminated tonic-clonic seizures in all animals (p<0.001). Conclusions: These findings suggest that Evolvulus alsinoides has anti-epileptic properties in PTZ-induced mice model.

Keywords: Evlolvulus alsinoides, PTZ, Diazepam, Epilepsy, Mouse model and morning glory

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Acute oral toxicity study of novel polyherbal formulations by using wistar rats and Swiss albino mice as per OECD 425 TG

Ramkishan Jatoth ^a,*, S.P Dhanabal ^b , V. Senthil ^c , T. Ganesh ^d, Jubie Selvaraj ^e, P.S. Venkatesan ^f

Abstract:

Background: Hydaljss08 is a new semisolid fermented hydroalcoholic polyherbal formulation containing crude drug substances. Immunoboost Tab is a novel polyherbal dosage form that is incorporated isolated, and fractionated phytochemical substances. Acute oral toxicity is a primary safety profile of the novel polyherbal for-mulations. As a part of our study, we estimated acute toxicity safety profile of novel dosage forms by a Swiss albino mice and Wistar rats.

Aim: According to the OECD 425 test guideline., the study aims to estimate acute toxicity tests of the “Hydaljss08,and Immunoboost Tab” dosage forms in rats and mice to assess the safety profile. Mice and rats are normally used in safety studies because of their susceptibility to toxic challenges, tumor induction, and carcinogenicity.

Material and Methods: 1 % CMC, Hydaljss08, Immunoboost-Tab polyherbal formulations, female rats, and albinomice. Animals were divided into the control groups, and treated groups with test dosage forms. At the end of the study, animals were euthanized by using the CO2 asphyxiation technique and subjected to gross pathological examinations.

Results: According to the assessment of the experimental data, the single-dose acute toxicity study of Hydaljss08 and Immunoboost-Tab formulations in mice and rats was fine. No signs of toxicity were observed in the animals treated at (2000 mg/kg.b. wt, Po), and revealed dullness, causing lethargy, and piloerections, which only per-sisted on the 1st day. At the end of the study, mild congestion in the liver, and lungs was observed in animals, treated with “Hydaljss08 formulation” compared to the vehicle control groups.

Conclusions: The estimated LD50 of Hydaljss08, and Immunoboost-Tab polyherbal formulations in rats and mice by oral route was found >2000 mg/kg, b.wt.

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T. taeniaeformis Infestation in Laboratory Rat

P.S. Venkatesan, M.Eswarya and M.Madhavaselvi

Abstract:

We report the infection of Cysticercus fasciolaris(C. fasciolaris), the larval form of Taenia taeniaeformis (T. taeniaeformis) in one of the rats used in a experiment in lab rats (Wistarrat). Animal showed symptoms like weight loss, lethargy, and a visibly poor coat condition. Gross Pathology revealed a pale and soft cyst in liver which was approximately 1.5 cm in diameter. Inside the cyst revealed a pale white segmented tapeworm (Taeniataeniaeformis). Histopathology of liver showed parasitic cyst surrounded by dense granulation tissue compress to the adjacent hepatocytes. Also few placed granulation tissue surrounding the fatty tissue compress the adjacent hepatocytes observed.

Keywords : Taenia taeniaeformis, Rat, Tapeworm, Liver cyst Taenia taeniaformis is a helminth parasite with cosmopolitan distribution, is found in the intestine of cat and related carnivores. Development of metacestode, Cysticercus fasciolaris occurs in the liver of rodents most often in mice and rats. C. fasciolaris in laboratory mice and rats by contaminated fees and bedding materials has been reported (Sharma et al., 2017). Once the rats have swallowed the eggs, the embryo develops into the earliest larval stage, Cysticercus fasciolaris. Hooks are used to poke rats’ muscle tissues and dig into the liver via the gut and bloodstream. Later, the larvae will reach the stage of strobilocerci. At this stage, fluid-filled cysts are formed, consisting of a scolex that attaches to the mucosa and connects to the terminal bladder until the larvae grow.

Conclusion: The study at Mass Biotech Chengalpet identified a Taenia taeniaeformis infestation in rats, which was characterized by a cystic liver and a tapeworm. Histopathology indicated the presence of Cysticercus fasciolaris, which caused significant liver damage. This unusual infection emphasizes the importance of increased bio security and regular health checks in preventing and treating parasite infections efficiently.

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Evaluation of antiepileptic properties of herbal mix of different
combinations by PTZ-induced mouse model

Evaluation of Anti-Epileptic Properties of Ashwagandha and Chamomile by
PTZ-Induced Mouse Model

Evaluation of anti-epileptic properties of Evolvulus alsinoides by pentylenetetrazole-induced mouse model

Acute oral toxicity study of novel polyherbal formulations by using wistar rats and Swiss albino mice as per OECD 425 TG

T. taeniaeformis Infestation in Laboratory Rat

MASS BIOTECH

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PUBLICATIONS

Evaluation of antiepileptic properties of herbal mix of different combinations by PTZ-induced mouse model

Evaluation of Anti-Epileptic Properties of Ashwagandha and Chamomile by PTZ-Induced Mouse Model

Evaluation of anti-epileptic properties of Evolvulus alsinoides by pentylenetetrazole-induced mouse model

Acute oral toxicity study of novel polyherbal formulations by using wistar rats and Swiss albino mice as per OECD 425 TG

T. taeniaeformis Infestation in Laboratory Rat

MASS BIOTECH

QUICK LINKS

QUICK LINKS

Contact Us

Evaluation of antiepileptic properties of herbal mix of different
combinations by PTZ-induced mouse model

Evaluation of Anti-Epileptic Properties of Ashwagandha and Chamomile by
PTZ-Induced Mouse Model